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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
12/12/2017 |
Data da última atualização: |
02/05/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MACEDO, L. L. P.; SOUZA JUNIOR, J. D. D. de; COELHO, R. R.; FONSECA, F. C. A.; FIRMINO, A. A. P.; SILVA, M. C. M.; FRAGOSO, R. R.; ALBUQUERQUE, E. V. S.; SILVA, M. S.; ENGLER, J. de A.; TERRA, W. R.; GROSSI-DE-SA, M. F. |
Afiliação: |
LEONARDO LIMA PEPINO DE MACEDO, CENARGEN; J. D. ANTONINO DE SOUZA JUNIOR, UNB; R. R. COELHO, UNB; F. C. A. FONSECA, UNB; A. A. P. FIRMINO, UFRGS; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; RODRIGO DA ROCHA FRAGOSO, CPAC; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN; MARILIA SANTOS SILVA, CENARGEN; J. DE ALMEIDA ENGLER, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE; W. R. TERRA, USP; MARIA FATIMA GROSSI DE SA, CENARGEN. |
Título: |
Knocking down chitin synthase 2 by RNAi is lethal to the cotton boll weevil. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Biotechnology Research and Innovation, v. 1, p. 72-86, 2017. |
DOI: |
10.1016/j.biori.2017.04.001 |
Idioma: |
Inglês |
Conteúdo: |
The cotton boll weevil (Anthonomus grandis) is the most destructive cotton insect pest in Brazil. The endophytic habit of this insect makes difficult its chemical control. Chitin synthase (CHS) is an integral membrane glycosyltransferase that is essential for chitin chain polymerization and deposition in insect chitinous structures, such as the peritrophic membrane (PM). Because it is not present in plants or vertebrates, CHS can be considered a promising target for eco-friendly biotechnological approaches, such as RNA interference (RNAi)-mediated gene silencing. Considering the relevance of CHS genes in the chitin biosynthetic pathway in insects, we report here the molecular cloning of the full-length CHS2 cDNA from the cotton boll weevil, and its functional validation via RNAi. The AgraCHS2 cDNA sequence is 4,869 bp, with a 4,446 bp open reading frame that encodes a predicted protein with 1,482 amino acid residues. Predicted protein has high similarity (53 to 78%) with other insects CHS. Moreover, only one copy is present in A. grandis genome. Transcriptional analysis showed that AgraCHS2 transcripts are restricted to the insect midgut at the third-larval instar and adult stages, which are considered the main feeding stages. RNAi-mediated knockdown of the AgraCHS2 affected A. grandis development, resulting in oviposition reduction of 93% and leading to 100% adult mortality. These data, in addition to the observation of PM severe disorganization in the midgut after AgraCHS2 knock-down, suggest AgraCHS2 as a promising target for developing RNAi-based biotechnological alternatives to specifically control the cotton boll weevil. MenosThe cotton boll weevil (Anthonomus grandis) is the most destructive cotton insect pest in Brazil. The endophytic habit of this insect makes difficult its chemical control. Chitin synthase (CHS) is an integral membrane glycosyltransferase that is essential for chitin chain polymerization and deposition in insect chitinous structures, such as the peritrophic membrane (PM). Because it is not present in plants or vertebrates, CHS can be considered a promising target for eco-friendly biotechnological approaches, such as RNA interference (RNAi)-mediated gene silencing. Considering the relevance of CHS genes in the chitin biosynthetic pathway in insects, we report here the molecular cloning of the full-length CHS2 cDNA from the cotton boll weevil, and its functional validation via RNAi. The AgraCHS2 cDNA sequence is 4,869 bp, with a 4,446 bp open reading frame that encodes a predicted protein with 1,482 amino acid residues. Predicted protein has high similarity (53 to 78%) with other insects CHS. Moreover, only one copy is present in A. grandis genome. Transcriptional analysis showed that AgraCHS2 transcripts are restricted to the insect midgut at the third-larval instar and adult stages, which are considered the main feeding stages. RNAi-mediated knockdown of the AgraCHS2 affected A. grandis development, resulting in oviposition reduction of 93% and leading to 100% adult mortality. These data, in addition to the observation of PM severe disorganization in the midgut after AgraCHS2... Mostrar Tudo |
Palavras-Chave: |
Insect pest control; Membrane; Peritrophic; RNAi (RNA interference). |
Thesaurus Nal: |
Midgut. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168697/1/1-s2.0-S2452072117300096-main.pdf
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Marc: |
LEADER 02586naa a2200325 a 4500 001 2082142 005 2024-05-02 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1016/j.biori.2017.04.001$2DOI 100 1 $aMACEDO, L. L. P. 245 $aKnocking down chitin synthase 2 by RNAi is lethal to the cotton boll weevil. 260 $c2017 520 $aThe cotton boll weevil (Anthonomus grandis) is the most destructive cotton insect pest in Brazil. The endophytic habit of this insect makes difficult its chemical control. Chitin synthase (CHS) is an integral membrane glycosyltransferase that is essential for chitin chain polymerization and deposition in insect chitinous structures, such as the peritrophic membrane (PM). Because it is not present in plants or vertebrates, CHS can be considered a promising target for eco-friendly biotechnological approaches, such as RNA interference (RNAi)-mediated gene silencing. Considering the relevance of CHS genes in the chitin biosynthetic pathway in insects, we report here the molecular cloning of the full-length CHS2 cDNA from the cotton boll weevil, and its functional validation via RNAi. The AgraCHS2 cDNA sequence is 4,869 bp, with a 4,446 bp open reading frame that encodes a predicted protein with 1,482 amino acid residues. Predicted protein has high similarity (53 to 78%) with other insects CHS. Moreover, only one copy is present in A. grandis genome. Transcriptional analysis showed that AgraCHS2 transcripts are restricted to the insect midgut at the third-larval instar and adult stages, which are considered the main feeding stages. RNAi-mediated knockdown of the AgraCHS2 affected A. grandis development, resulting in oviposition reduction of 93% and leading to 100% adult mortality. These data, in addition to the observation of PM severe disorganization in the midgut after AgraCHS2 knock-down, suggest AgraCHS2 as a promising target for developing RNAi-based biotechnological alternatives to specifically control the cotton boll weevil. 650 $aMidgut 653 $aInsect pest control 653 $aMembrane 653 $aPeritrophic 653 $aRNAi (RNA interference) 700 1 $aSOUZA JUNIOR, J. D. D. de 700 1 $aCOELHO, R. R. 700 1 $aFONSECA, F. C. A. 700 1 $aFIRMINO, A. A. P. 700 1 $aSILVA, M. C. M. 700 1 $aFRAGOSO, R. R. 700 1 $aALBUQUERQUE, E. V. S. 700 1 $aSILVA, M. S. 700 1 $aENGLER, J. de A. 700 1 $aTERRA, W. R. 700 1 $aGROSSI-DE-SA, M. F. 773 $tBiotechnology Research and Innovation$gv. 1, p. 72-86, 2017.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
12/11/2015 |
Data da última atualização: |
12/11/2015 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
NOGUEIRA, C. H. P.; CORREIA, N. M.; GOMES, L. J. P.; FERREIRA, P. S. H. |
Afiliação: |
CASSIO HENRIQUE PEREIRA NOGUEIRA, UNESP-FCAV; NUBIA MARIA CORREIA, CNPH; LEONARDO JOSÉ PETEAN GOMES, UNESP-FCAV; PHELLIPE SANT'ANNA HONORIO FERREIRA, UNESP-FCAV. |
Título: |
Seletividade dos herbicidas bentazon e nicosulfuron para crotalaria juncea e crotalaria spectabilis. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FITOSSANIDADE, 3., 2015, Aguas de Lindódia. Anais... São Paulo, SP: UNESP, 2015. 1 CD-ROM. |
Páginas: |
p. 189-193. |
Idioma: |
Português |
Conteúdo: |
O cultivo de crotalária apresenta vários benefícios, porém, são escassas as informações na literatura para o sistema de consórcio com milho, principalmente quanto à seletividade de herbicidas utilizados no milho para plantas de crotalária. Objetivou-se estudar a seletividade dos herbicidas nicosulfuron e bentazon para Crotalaria juncea e Crotalaria spectabilis, quando pulverizados em pós-emergência das plantas. |
Palavras-Chave: |
Basagran; Sanson. |
Thesagro: |
Adubação; Agrotóxico; Erva daninha. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/132916/1/Nubia-5-47.pdf
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Marc: |
LEADER 01141nam a2200217 a 4500 001 2028420 005 2015-11-12 008 2015 bl uuuu u00u1 u #d 100 1 $aNOGUEIRA, C. H. P. 245 $aSeletividade dos herbicidas bentazon e nicosulfuron para crotalaria juncea e crotalaria spectabilis.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE FITOSSANIDADE, 3., 2015, Aguas de Lindódia. Anais... São Paulo, SP: UNESP, 2015. 1 CD-ROM.$c2015 300 $ap. 189-193. 520 $aO cultivo de crotalária apresenta vários benefícios, porém, são escassas as informações na literatura para o sistema de consórcio com milho, principalmente quanto à seletividade de herbicidas utilizados no milho para plantas de crotalária. Objetivou-se estudar a seletividade dos herbicidas nicosulfuron e bentazon para Crotalaria juncea e Crotalaria spectabilis, quando pulverizados em pós-emergência das plantas. 650 $aAdubação 650 $aAgrotóxico 650 $aErva daninha 653 $aBasagran 653 $aSanson 700 1 $aCORREIA, N. M. 700 1 $aGOMES, L. J. P. 700 1 $aFERREIRA, P. S. H.
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